Ask The Brewmasters

  • 1.  Looking Sequencing Identification Lab

    This message was posted by a user wishing to remain anonymous
    Posted 03-06-2023 15:20
    This message was posted by a user wishing to remain anonymous

    Hello all,

    I am looking for a lab for sequence identification of bacterial/fungus isolates. I remember stumbling across a link in the last few months of a lab that does sequencing identification at a reasonable price. It was like $40 a sample for 16S on isolated colonies. I thought it was an Ask the Brewmasters post but I cannot find the link (stupidly forgot to bookmark it). It may have been in a webinar, poster, or ASBC source. 

    Thanks,



  • 2.  RE: Looking Sequencing Identification Lab

    Posted 03-07-2023 12:09

    Try Midi Labs.

    https://www.midilabs.com/



    ------------------------------
    Jason Weihbrecht
    GM of Brewery Operations
    Oskar Blues Brewery
    Brevard NC
    ------------------------------



  • 3.  RE: Looking Sequencing Identification Lab

    Posted 03-07-2023 12:10

    We use MIDI labs, who recently became part of Biolog:

    https://www.biolog.com/

    They are affordable, and we have been happy with their service.

     

    Cullen Dwyer

    Q/A Manager

    P: 978.874.9965 X1160


    Wachusett Brewing Company, Inc.
    175 State Road E.
    Westminster, MA  01473

    www.wachusettbrewingcompany.com

     






  • 4.  RE: Looking Sequencing Identification Lab

    Posted 03-08-2023 12:21
    Edited by Andy Tveekrem 03-08-2023 18:57
    I have used genewiz in the past, affordable and quick.




    Original Message


  • 5.  RE: Looking Sequencing Identification Lab

    Posted 03-07-2023 14:15

    Most yeast labs will offer something like this. I am pretty sure Bootleg Biology does. Not sure on prices.



    ------------------------------
    Dan Pixley
    Milk The Funk Wiki Editor
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  • 6.  RE: Looking Sequencing Identification Lab

    Posted 03-07-2023 20:06
    I currently am starting to use Eurofins - Good service and support 800-651-4802

    Jeff

    Jeff Gunn
    President & CEO
    IDD Process & Packaging, Inc.
    5450 Tech Circle
    Moorpark, CA 93021
    Toll Free: 800-621-4144
    Tel: 805-529-9890
    Fax: 805-529-9282
    Cell: 805-444-3842
    Web Site: www.iddeas.com





  • 7.  RE: Looking Sequencing Identification Lab

    Posted 03-08-2023 16:14

    Look into Genewiz (now Azenta Life Sciences) for 16S (bacterial) and ITS (fungal) sequencing. It is very affordable (I think $18/samples) and pretty straightforward. You can send a plate with isolated colonies and they will prepare the DNA, PCR and sanger sequence. For 16S they will provide analysis and a predicted bacterial species from the 16S sequence. For ITS, you can run a blast on the sequencing results with NCBI and that will get you a good idea of the fungal species.

    -Laura



    ------------------------------
    Laura Burns
    Director of Research and Development
    Omega Yeast
    Chicago IL
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  • 8.  RE: Looking Sequencing Identification Lab

    Posted 03-09-2023 12:06

    We use Eton Biosciences: https://www.etonbio.com/



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    Ashley Howard
    Victory Brewing Company
    Parkesburg PA
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  • 9.  RE: Looking Sequencing Identification Lab

    Posted 03-09-2023 16:36

    Our laboratory used to be next door to Eton Bio here in Research Triangle Park, NC. They are a small, high-powered team with fast, reliable service.



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    Volker Bornemann
    President
    Avazyme, Inc.
    Research Triangle Park, NC
    avazyme.com
    ------------------------------



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