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  • 1.  Anaerobic incubation temperature

    Posted 02-13-2024 15:09

    Hello all,

    Wondering what temperature others are incubating their anaerobic plates at... 21°C? 28°C? 35°C?

    Thank you,

    Adam Donkers



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    Adam Donkers
    National Microbiologist and Food Safety Specialist
    Sleeman Brewing
    Guelph ON
    (519) 831-4666
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  • 2.  RE: Anaerobic incubation temperature

    Posted 02-14-2024 13:01

    Hi Adam,

      We usually target between 32C and 37C, depending on the type of media and organisms you are looking for.

    Kory



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    Kory Davis
    QC and Propagation Specialist
    The Brewing Science Institute
    Woodland Park, Co
    719-482-4895 ext. 3
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    Original Message


  • 3.  RE: Anaerobic incubation temperature

    Posted 02-14-2024 14:31

    I've always used 28°C for aerobic and anaerobic plates. I think we had a small incubator at 35°C that was only used for testing process water samples. 



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    Lauren Behrmann
    Technical Brewing Manager
    Mark Anthony Brewing
    Fort Worth TX
    lbehrmann@mabrewing.com
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    Original Message


  • 4.  RE: Anaerobic incubation temperature

    Posted 02-15-2024 16:00

    General recommendation for incubation temperature according to EBC methods is 25 degrees plus/minus 2.

    This temperature is good for lactic acid bacteria as well as for the strict anaerobes and wild yeast growth.

    Only for diastaticus specific testing, higher temperatures of 30 degrees up to 37 degrees are recommended.

    Some lactics and also yeasts won't grow at 30 degrees and higher.



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    Gudrun Vogeser
    Founder & Owner
    PIKA Weihenstephan
    Pfaffenhofen, Bavaria
    +49 8441 8794830
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    Original Message


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